Strategies for evaluation and amplification of hematopoietic stem cells proliferative potential – therapeutics applications

Principal investigator: Mihaela Economescu (Chivu) PhD

(2004 -2006; VIASAN 343) – Principal investigator: Mihaela Economescu (Chivu) PhD

Funding: 25000 Euro

Collaborator: Clinic Hospital Dr. I. Cantacuzino, Bucharest, Romania

Activities:

Phase I – Analysis of protocols for the separation of hematopoietic stem cells to enhance their performance.

Phase II – Evaluation of the impact of maternal variables on prenatal pathology.

Phase III – Optimization of culture systems for hematopoietic stem cells while maintaining self-regeneration and differentiation properties.

Phase IV – Determination of the susceptibility of hematopoietic stem cells to viral infections and their capacity to sustain viral replication.

Phase V – In vitro testing of the effects of antitumor and antiviral drugs on hematopoiesis.

Results:

Mihaela Chivu, Carmen C. Diaconu, Coralia Bleotu, Irina Alexiu, Lorelei Brasoveanu, C. Cernescu, The comparison of different protocols for expansion of umbilical cord blood hematopoietic stem cell, J. Cell. Mol. Med., 2004, Vol 8, No 2,  223-231.

Mihaela Chivu, Serban Nastasia, Camelia Sultana, Coralia Bleotu, Irina Alexiu, Decebal Hudita,Optimizing donor selection in order to establish a cord blood banking facility: maternal and obstetric factors impact, Central European Journal of Medicine, 2007, Vol 2, No 2, 180-189.