Diagnosis markers and management criteria for human papilomavirus cervix infection

Acronym: VIROGYN
Principal Investigator Gabriela Anton, PhD.

(VIROGYN; 2006-2008); Principal Investigator Gabriela Anton, PhD.

Aim: To establish the prevalence and the incidence of the HPV genotypes circulating in different area of Romania; to correlate the obtained results in order to improve the monitoring of the lesions and management of the viral infection

Partners: Coordinator: „Stefan S. Nicolau” Institute of Virology – project director Gabriela Anton, PhD

Partner 1 – Filantropia Clinical Hospital, Bucharest, responsible Prf Gheorghe Peltecu, PhD

Partner 2 – „Grigore T. Popa” University Of Medicine and Pharmacy, Iasy, responsible Demetra Socolov PhD

Partner 3 – University Of Medicine and Pharmacy, Craiova, responsible Florin Cornitescu PhD

Partner 4 – „Carol Davila” University of Medicine and Pharmacy, Bucharest, responsible Mariana anton PhD

Partner 5 – Fundeni Clinical Institute, Bucharest, responsible Ileana Constantinescu PhD

Partner 6 – University of Bucharest, responsible Marieta Costache PhD

Working team: Anton G, Antipa C, Diaconu C, Plesa A, Stoian M, Manolescu L, Bleotu C, Dragodan V, Zaharia C

Funding: IVN=602 980 LEI

Project CEEX – M1- 119/2006

Implementation period: 09.2006 – 11.2008

Summary: The molecular epidemiologic data showed in an unequivocal manner that the human papillomaviruses (HPVs) are the etiologic agent of this disease. There is not a consensus regarding the inclusion of some genotypes with low prevalence in a special risk group. The genital infection with HPV must be managed as a higher risk (Hr) factor which predispose to the cervical cancer development. In this infection, the certainly diagnosis is done by PCR, so, it is not surprisingly that at the international, but also the national level, there are numerous studies focused on the value of the high specificity grade enabled by these assays, in order to eliminate cyto/colposcopic discordances. It is generally admitted that the HrHPV infection is not sufficient for the cervical cancer pathogenesis, other complementary conditions being involved The reason that certain infections are persistent may depend on: the inter-individual variations in the capacity to elicit an adequate immune response; the persistence and the clonal viral heterogeneity; – the viral oncogenes interference with the tumour suppressor proteins; the epigenetic events.

In this context we intend to verify the accuracy of the cytologic diagnosis on thin layer preparations ( Thin Prep), as compared with classical techniques; to evaluate the consistency relation between the immunocytochemical determination of the Hr HPV L1 proteins and the PCR detection of the corresponding viral genome, in order to monitories the evolution; to quantify the expression of some cell and viral genes involved into the inter-individual variations of the immune response, in the viral persistence and in the progression of the genital lesions;


  1. Adriana Plesa, Cristina Goia, Anca Botezatu, Coralia Bleotu, Demetra Socolov, Gheorghe Peltecu, Florin Cornitescu, Gabriela Anton, Analysis of HPV DNA and p16 expression to predict prognosis in ASCUS, LSIL and HSIL in cervical swabs, 33rd FEBS Congress and 11th IUBMB Conference, Biochemistry of Cell Regulation, Athens, Greece, 28 june- 3 july 2008, FEBS Journal 275 (Suppl. 1) 99-437 (2008), p. 274; ISSN: 1742-4658. IF=3.39
  2. Marina Anton, Gabriela Anton, Zorela Sgarbura, D. Iliescu, Coralia Bleotu, Anca Botezatu, Cristina Goia, Adriana Plesa, E6 human papillomavirus type 16 methylation in clinical specimens, 33rd FEBS Congress and 11th IUBMB Conference, Biochemistry of Cell Regulation, Athens, Greece, 28 iunie- 3 iulie 2008, FEBS Journal 275 (Suppl. 1) 99-437 (2008), p. 102. ISSN: 1742-4658. IF=3.39
  3. Gabriela Anton, Demetra Socolov, A.C. Anton, Coralia Bleotu, Marina Anton, F. Cornitescu, S. Teleman, D. Iliescu, Anca Botezatu, Cristina Goia, Adriana Plesa, Viral persistence- factor of cervical lesions progression, HPV in Human pathology, Prague, Chech Republic, 1-3 may 2008, abstract in Central European Journal of Public Health, vol. 16 (JHEMI vol. 52), p26, S61, April 2008; ISSN 1210-7778 (Print) IF=1.481