The cell culture laboratories offer the capabilities to conduct a wide range of experiments, including isolating cells from blood and tissues, establishing and characterizing primary cell cultures and cancer stem cells, immortalizing cells, and maintaining 2D and 3D cell cultures, cell lines, and biobanking. They can also conduct virus isolation and identification assays, as well as real-time monitoring of cellular processes such as cell proliferation, cytotoxicity, adhesion, viability, invasion, and migration. The labs can perform transfections and gene silencing using siRNA or shRNA, and retroviral vector transductions. Additionally, they conduct in vitro testing of anti-tumoral and virucidal activity using high throughput (HTS) evaluation.
Incucyte S1 Live-Cell Imaging and Analysis – Essen Bioscience – Sartorius
Inverted Microscope for Cell Culture for transmitted-light brightfield, phase contrast and camera DMi1 – Leica
Microscope Axio Observer.D1 for transmitted-light brightfield, phase contrast and fluorescence – Zeiss
Allegra X-12R, Benchtop Centrifuge, Refrigerated – Beckman Coulter
Low (-300C) and ultralow (-800C) freezers
xCELLigence RTCA DP Instrument
Laser Microdissection Systems LMD7000 – Leica
Water-jacked incubators Series 8000 – Thermo Scientific
Laminar flow cabinets – Class II
MiliQ water system
